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β‐Elimination of phosphate and subsequent addition of pyridoxamine as a method for identifying and sequencing peptides containing phosphoseryl residues
Author(s) -
Hastings Teresa G.,
Reimann Erwin M.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80865-2
Subject(s) - edman degradation , chemistry , pyridoxamine , peptide , chromatography , residue (chemistry) , adduct , phosphate , biochemistry , absorbance , fluorescence , peptide sequence , organic chemistry , quantum mechanics , gene , pyridoxal , physics
Peptides containing phosphoseryl residues can be modified by removal of the phosphate groups via β‐elimination followed by addition of pyridoxamine to the resulting dehydroalanyl residue. Peptides containing the modified residues can be detected at nanomole levels by monitoring absorbance at 328 nm or at picomole levels by monitoring fluorescence. Photolysis of the modified peptide converts the pyridoxamino adduct to a form which can be readily identified after Edman degradation.