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Phosphorylation of the 90 kDa heat shock protein in heat shocked HeLa cell lysates
Author(s) -
Legagneux Vincent,
Dubois Marie-Françoise,
Morange Michel,
Bensaude Olivier
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80862-7
Subject(s) - hela , heat shock protein , phosphorylation , kinase , incubation , protein kinase a , hspa14 , in vitro , microbiology and biotechnology , biochemistry , chemistry , biology , hsp70 , gene
The 90 kDa heat shock protein (hsp 90) is a major phosphorylated protein under normal growth conditions. However, it does not incorporate detectable levels of phosphate by incubation of control HeLa cell lysates with [γ‐ 32 P]ATP in vitro. In this paper we show that strong phosphorylation of hsp 90 occurs in lysates prepared from heat shocked HeLa cells. Possible involvement of the eukaryotic initiation factor 2 kinase of the heme‐controlled repressor of translation is discussed.