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Modulation of HLA antigens in response to the binding of epidermal growth factor by A431 cells
Author(s) -
Hosoi Kazuo,
Kittur Dilip S.,
Edidin Michael
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80852-4
Subject(s) - epidermal growth factor , antigen , flow cytometry , a431 cells , phosphorylation , human leukocyte antigen , biology , microbiology and biotechnology , cell culture , chemistry , cell , cancer research , immunology , biochemistry , cell cycle , oncogene , genetics
In a previous study [(1984) J. Cell Biol. 98, 725–731] we showed that the level of human MHC, HLA antigens on A431 carcinoma cells is reduced after these cells bind epidermal growth factor (EGF). Here we use flow cytometry to determine the effects of various doses and times of EGF treatment on HLA expression. We then show that the reduction in HLA expression is associated with a reduction in the level of phosphorylation of immunoprecipitable surface HLA antigens, although longer exposure of cells with EGF increased both surface HLA expression and their phosphorylation levels. Lateral diffusion of HLA antigens is lower in EGF‐treated than in control cells. The lower diffusion coefficients measured may be causally related to the decreased phosphorylation of HLA antigens.