Premium
A zebrafish engrailed‐like homeobox sequence expressed during embryogenesis
Author(s) -
Fjose Anders,
Eiken Hans G.,
Njølstad Pål R.,
Molven Anders,
Hordvik Ivar
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80849-4
Subject(s) - homeobox , engrailed , zebrafish , homeobox protein nkx 2.5 , embryogenesis , sequence (biology) , biology , microbiology and biotechnology , homeobox a1 , computational biology , genetics , gene , embryo , gene expression
The zebrafish genome was found to contain two sequences which cross‐hybridize strongly with the engrailed gene of Drosophila . Several independent clones containing one of these cross‐hybridizing sequences were isolated from a zebrafish genomic library. Characterization of this region (ZF‐EN) by DNA sequencing showed that it shares about 70% sequence identity with the engrailed homeobox. More extensive homeobox homology (90%) was found relative to the murine En genes. The closest relationship exists between ZF‐EN and En ‐2 where the C‐terminal domains (104 amino acids) encoded by these genes are almost identical. We also observed that ZF‐EN and En‐2 are very similar with respect to their transcript sizes and temporal expression patterns.