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Sequence‐specific chemical modification of a hybrid bacteriophage M 13 single‐stranded DNA by alkylating oligonucleotide derivatives
Author(s) -
Vlassov V.V.,
Zarytova V.F.,
Kutyavin I.V.,
Mamaev S.V.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80848-2
Subject(s) - oligonucleotide , dna , bacteriophage , sequence (biology) , nucleic acid sequence , nucleotide , dna sequencing , chemistry , biology , microbiology and biotechnology , combinatorial chemistry , biochemistry , gene , escherichia coli
Alkylating oligonucleotide derivatives react with the complementary sequences in hybrid M 13mp7 bacteriophage single‐stranded DNA and destroy the infecting ability of the DNA. The reagents do not damage M 13mp9 single‐stranded DNA lacking the target nucleotide sequence.