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Nucleotide and primary sequence of a major rice prolamine
Author(s) -
Kim Woo Taek,
Okita Thomas W.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80839-1
Subject(s) - biology , complementary dna , open reading frame , homology (biology) , genetics , protein primary structure , clone (java method) , cdna library , nucleic acid sequence , peptide sequence , gene
A recombinant cDNA clone encoding a major rice seed storage prolamine was isolated by antibody screening of a cDNA λgt 11 library. This clone contained a single open reading frame encoding a putative rice prolamine precursor ( M r 17 300). In contrast to other cereal prolamines, the primary sequence of the rice prolamine was devoid of any major tandem repetitive sequences, a feature prevalent in all cereal prolamines studied to date. No significant homology was detected between the rice prolamine and other cereal prolamines, indicating that the rice gene evolved from unique ancestral DNA segments.