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A 5 kb intergenic region containing oril in the mitochondrial DNA of Saccharomyces cerevisiae is dispensable for expression of the respiratory phenotype
Author(s) -
Piškur Jure
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80815-9
Subject(s) - intergenic region , saccharomyces cerevisiae , mitochondrial dna , phenotype , genetics , biology , dna , microbiology and biotechnology , gene , genome
A mutant mitochondrial DNA of Saccharomyces cerevisiae strain R 0.54 has been shown to have a 5 kb deletion which removes the oril sequence and its surrounding intergenic sequences between the pro tRNA and 15 S rRNA genes. The deleted DNA has been sequenced and is found to be flanked by two 11 bp direct repeats. The deletion mutant displays a normal phenotype in non‐fermentable media. This implies that the intergenic sequence is not required for expression of the respiratory phenotype.