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Psoralen photofootprinting of protein‐binding sites on DNA
Author(s) -
Zhen Wei-ping,
Jeppesen Claus,
Nielsen Peter E.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80800-7
Subject(s) - psoralen , chemistry , dna , biophysics , biochemistry , biology
Using a BAL31 exonuclease assay to determine the sites of 4,5′,8‐trimethylpsoralen photocrosslinking in DNA we have shown that 5′‐TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, λ‐repressor E. coli or RNA polymerase are bound at their recognition DNA sequences (O R1 operator or deol promoter, respectively). These results show that psoralens can be used as photofootprinting reagents to study specific protein‐DNA interactions.