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(Ca 2+ + Mg 2+ )‐dependent ATPase mRNA from smooth muscle sarcoplasmic reticulum differs from that in cardiac and fast skeletal muscles
Author(s) -
de la Bastie Diane,
Wisnewsky Claudine,
Schwartz Ketty,
Lompré Anne-Marie
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80794-4
Subject(s) - endoplasmic reticulum , skeletal muscle , atpase , chemistry , cardiac muscle , messenger rna , biophysics , enzyme , endocrinology , biochemistry , biology , gene
We have investigated some characteristics of the sarcoplasmic reticulum (Ca 2+ + Mg 2+ )‐dependent ATPase (Ca 2+ ‐ATPase) mRNA from smooth muscle using specific cDNA probes isolated from a rat heart cDNA library. RNA blot analysis has shown that the Ca 2+ ‐ATPase mRNA expressed in smooth muscle is identical in size to the cardiac mRNA but differs from that of fast skeletal muscle. S 1 nuclease mapping has moreover shown that the cardiac and smooth muscle isoforms possess different 3′‐end sequences. These results indicate that a distinct sarcoplasmic reticulum Ca 2+ ‐ATPase mRNA is present in smooth muscle.