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Electroporation: High frequency of occurrence of a transient high‐permeability state in erythrocytes and intact yeast
Author(s) -
Weaver James C.,
Harrison Gail I.,
Bliss Jonathan G.,
Mourant Judith R.,
Powell Kevin T.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80791-9
Subject(s) - propidium iodide , electroporation , schizosaccharomyces pombe , yeast , population , membrane , biophysics , membrane permeability , chemistry , fluorescence , flow cytometry , macromolecule , intracellular , permeability (electromagnetism) , biochemistry , biology , saccharomyces cerevisiae , microbiology and biotechnology , apoptosis , programmed cell death , medicine , gene , physics , environmental health , quantum mechanics
We present the first determinations of population distributions of macromolecule uptake due to electroporation, the percentage of cells which participate and, for the yeast, the subpopulation of cells whose membranes exhibit significant recovery following macromolecule uptake. Flow cytometry is used to measure the uptake of a first test molecule (green fluorescence, FITC‐dextran; 70 kDa) and also, for the yeast, the subsequent uptake of a second, much smaller, test molecule (red fluorescence, propidium iodide; 660 Da), which provides a measure of membrane recovery. A dramatic 20% (erythrocytes) to 75% (intact Schizosaccharomyces pombe ) of cells can take up the first test molecule within 5 min of a pulse.