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Do DNA polymerases δ and α act coordinately as leading and lagging strand replicases?
Author(s) -
Focher Federico,
Ferrari Elena,
Spadari Silvio,
Hübscher Ulrich
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80786-5
Subject(s) - polymerase , dna polymerase , dna , lagging , enzyme , cytoplasm , nucleus , biochemistry , dna replication , dna clamp , biology , microbiology and biotechnology , chemistry , gene , polymerase chain reaction , reverse transcriptase , medicine , pathology
The activity ratio of DNA polymerases δ and α in calf thymus was found to be invariably 1:1, irrespective of extraction procedure (8 types) and subcellular localization (cytoplasm, nucleus and microsomes). This was established by separation of the two forms by hydroxyapatite chromatography and by their response to specific inhibitors and monoclonal antibodies. This finding supports the dimeric DNA polymerase model [(1980) J. Biol. Chem. 255, 4290–4303], which proposes that DNA polymerases δ and α act coordinately as leading and lagging strand enzymes, respectively, at the replication fork.