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Microinjection of catalytic subunit of cyclic AMP‐dependent protein kinases triggers acute morphological changes in thyroid epithelial cells
Author(s) -
Roger Pierre P.,
Rickaert Fabienne,
Huez Georges,
Authelet Michèle,
Hofmann Franz,
Dumont Jacques E.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80781-6
Subject(s) - microinjection , protein subunit , protein kinase a , kinase , microbiology and biotechnology , chemistry , biochemistry , biology , gene
In dog thyroid epithelial cells in primary culture, thyrotropin acting through cyclic AMP induced rapid morphological changes associated with complete disruption of actin containing stress fibers. This modification preceded cell retraction and rounding up. These morphological effects were also induced by glass capillary microinjection of purified catalytic subunit of cAMP‐dependent protein kinase. This provides the first direct evidence in intact cells that catalytic subunit, which is released upon activation of cAMP‐dependent protein kinases, is responsible for cAMP‐dependent morphological transformation.