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Linolenic acid‐induced release of Mn, polypeptides and inactivation of oxygen evolution in photosystem II particles
Author(s) -
Garstka Maciej,
Kaniuga Zbigniew
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80772-5
Subject(s) - photosystem ii , incubation , chemistry , oxygen evolution , urea , peptide , manganese , oxygen , biochemistry , photosynthesis , organic chemistry , electrochemistry , electrode
The release of both Mn and polypeptides as well as the inactivation of O 2 evolution in C 18:3 ‐treated PS II particles was studied. An increase of incubation temperature above 20°C greatly accelerated depletion of both Mn and peptides and inactivation of O 2 evolution. At 25°C about 30% of Mn was released whereas following incubation at 30°C release of 50% of Mn, complete depletion of both 10 and 18 kDa peptides as well as complete inactivation of O 2 evolution were observed. Simultaneously, the release of 24 and both 33 and 43 kDa peptides was about 40 and 30%, respectively. At 25°C, C 18:3 released only one Mn/reaction centre from PS II particles depleted of 18 and 24 kDa peptides, while two Mn/reaction centre were released from particles devoid of 18, 24 and 33 kDa peptides. These observations suggest that the 33 kDa peptide stabilizes preferentially two of the four Mn atoms of the oxygen‐evolving complex. The effect of C 18:3 on Mn release from PS II particles is thus similar to that of heat and urea treatments.