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Phospholipid uptake by Plasmodium knowlesi infected erythrocytes
Author(s) -
Moll G.N.,
Vial H.J.,
Ancelin M.L.,
Op den Kamp J.A.F.,
Roelofsen B.,
van Deenen L.L.M.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80765-8
Subject(s) - phosphatidylethanolamine , plasmodium knowlesi , phosphatidylserine , phospholipid , phosphatidylcholine , phospholipid transfer protein , biochemistry , chemistry , parasite hosting , biology , membrane , plasmodium falciparum , malaria , world wide web , computer science , immunology , plasmodium vivax
The uptake of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS) in Plasmodium knowlesi infected erythrocytes has been studied. Whereas uptake of phospholipids, in the absence of phospholipid transfer proteins, is negligible in control cells, the infected cells can incorporate considerable amounts of added phospholipids. The uptake is enhanced by the presence of lipid transfer proteins. Doubly labeled [ 3 H]oleate, [ 14 C]choline) PC does not undergo any appreciable remodelling following uptake, which strongly suggests that plasma PC is used as such for the biogenesis of the parasite membranes. Transport of extracellularly offered PS and PE towards the intraerythrocytic parasite and utilization of these lipids by the parasite are confirmed by the observation that these lipids are converted into respectively PE and PC. The extent and rate of these conversions depend on the way the phospholipids are introduced into the infected cells.