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Cloning and characterization of the human gene for the α‐subunit of G i2 , a GTP‐binding signal transduction protein
Author(s) -
Weinstein L.S.,
Spiegel A.M.,
Carter A.D.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80764-6
Subject(s) - exon , biology , microbiology and biotechnology , gene , polyadenylation , 5' flanking region , tata box , primer extension , genetics , promoter , messenger rna , gene expression
We isolated and characterized human genomic clones encompassing the gene for the α‐subunit of G i2 , a GTP‐binding signal transduction protein abundantly expressed in myeloid cells. The gene is divided into 9 exons and spans 23.5 kb. Exons 2, 6 and 7 encode putative guanine nucleotide‐binding domains that are highly conserved among GTP‐binding proteins. A polyadenylation signal located within exon 9 predicts an MRNA size (∼ 2.3 kb) several hundred bases longer than that of published cDNAs, and consistent with the size seen on RNA blot hybridization. Primer extension and S 1 nuclease analysis determined a major and several minor transcriptional start sites. The first exon and 5′ flanking region are highly G+ C rich, contain several GC boxes (SP1 transcription factor binding sites), a CAAT box, and lack a TATA box. The presumptive promoter region is thus similar to that of ras and other widely expressed genes.