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Influence of 2‐chloroadenosine on the nucleotide content of isolated rat hepatocytes
Author(s) -
Claeyssens Sophie,
Hamet Michèle,
Chedeville Arlette,
Basuyau Jean-Pierre,
Lavoinne Alain
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80761-0
Subject(s) - nucleotide , chemistry , food science , microbiology and biotechnology , biology , biochemistry , gene
2‐Chloroadenosine is presumably a non‐metabolizable analogue of adenosine; however, this compound induced an increase in the enzymatically measured nucleotide content of isolated rat hepatocytes. HPLC separation and spectral analysis of the peaks showed that this increase may be related to the formation of 2‐chloro nucleotides and that the 2‐chloro nucleotides appeared in the first minutes of the incubation period. These results demonstrate that 2‐chloroadenosine may be metabolized by phosphorylation in rat liver cells.