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Sex‐related difference in oxidative metabolism of testosterone and erythromycin by hamster liver microsomes
Author(s) -
Miura Toshiaki,
Komori Masayuki,
Iwasaki Miyuki,
Kurozumi Kimikazu,
Ohta Kazuhide,
Ohmori Shigeru,
Kitada Mitsukazu,
Kamataki Tetsuya
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80727-0
Subject(s) - microsome , cytochrome , hamster , testosterone (patch) , biology , cytochrome p450 , endocrinology , medicine , microsoma , oxidative phosphorylation , western blot , mesocricetus , metabolism , biochemistry , enzyme , gene
The activities of testosterone hydroxylases and erythromycin N ‐demethylase were significantly higher in liver microsomes from female hamsters than in the male counterparts. SDS‐polyacrylamide gel electrophoresis revealed a difference in protein composition between male and female liver microsomes in the molecular mass region comprising cytochrome P‐450. Western blot analysis showed further that antibodies to rat male‐specific cytochrome P‐450 crossreacted with at least two proteins in both male and female hamster microsomes, but one of the female proteins had a different molecular mass from that of the male proteins. It is concluded that sex difference in liver microsomal cytochrome P‐450 is not restricted to rats and mice, as has previously been believed.