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Gene expression of fibroblast matrix proteins is altered by indomethacin
Author(s) -
Mauviel A.,
Kähäri V.-M.,
Heino J.,
Daireaux M.,
Hartmann D.J.,
Loyau G.,
Pujol J.-P.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80715-4
Subject(s) - fibronectin , fibroblast , procollagen peptidase , extracellular matrix , collagenase , chemistry , messenger rna , proteoglycan , radioimmunoassay , extracellular , protein biosynthesis , secretion , type i collagen , prostaglandin , medicine , endocrinology , biochemistry , gene , biology , enzyme , in vitro
The role of indomethacin in the regulation of extracellular matrix synthesis was studied in dermal fibroblast cultures. Indomethacin (10 μM) blocked totally the prostaglandin secretion and markedly increased the synthesis of collagen. In parallel, measurement of fibronectin, type I and type III procollagen mRNA levels showed a substantial increase under the action of indomethacin. On the other hand, indomethacin did not modify the mRNA levels of dermatan sulfate proteoglycan core protein. Measurement of collagen production estimated as the amount of collagenase digestible protein and by specific radioimmunoassay indicated a good correlation with the corresponding mRNA levels. These results suggest that indomethacin can regulate the extracellular matrix deposition at a transcriptional level.