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Evidence that insulin increases the proportion of polysomes that are bound to the cytoskeleton in 3T3 fibroblasts
Author(s) -
Hesketh John E.,
Pryme Ian F.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80703-8
Subject(s) - polysome , cytoskeleton , actin , insulin , microfilament , microbiology and biotechnology , chemistry , biochemistry , biology , biophysics , endocrinology , rna , ribosome , cell , gene
The association of polysome redistribution with changes in protein synthesis was investigated in insulin‐stimulated fibroblasts. Free polysomes were released by Nonidet‐P40 and 25 mM KCl, cytoskeletal‐bound polysomes were retained at 25 mM KCl but released at 130 mM, while membrane‐bound polysomes were released by deoxycholate. Insulin increased the proportion of polysomes which were retained at 25 mM KCl but had no effect when extraction was carried out at 130 mM KCl, suggesting that more polysomes were associated with the cytoskeleton. Insulin also reduced the amount of actin released from the detergent‐insoluble cytoskeleton indicating that the hormone affects microfilament organization.