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A dicarboxylate transporter on the peribacteroid membrane of soybean nodules
Author(s) -
Udvardi Michael K.,
Price G.Dean,
Gresshoff Peter M.,
Day David A.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80697-5
Subject(s) - transporter , membrane , biochemistry , chemistry , organic anion transporter 1 , biology , gene
Using preparations of peribacteroid membrane (PBM)‐enclosed bacteroids from soybean root nodules, we show here that the PBM possesses a dicarboxylate transporter capable of mediating a rapid flux of dicarboxylate anions, such as malate and succinate, to the bacteroids inside the nodule. The transporter has a higher affinity for the monovalent malate anion than for the succinate anion ( K m = 2 and 15 μM, respectively) although the V max for malate − appears to be lower than for succinate − ( V max = 11 and 30 nmol·min −1 ·mg protein −1 , respectively).