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Immunoprecipitation of human adrenal microsomal antigen
Author(s) -
Furmaniak J.,
Talbot D.,
Reinwein D.,
Benker G.,
Creagh F.M.,
Smith B.Rees
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80695-1
Subject(s) - immunoprecipitation , immunofluorescence , microsome , antibody , antigen , microbiology and biotechnology , chemistry , endocrinology , medicine , biology , immunology , biochemistry , enzyme
Human adrenal microsomes have been labelled with 125 I and immunoprecipitated with sera from patients with Addison's disease. The immunoprecipitates were then analysed by SDS‐PAGE and autoradiography. 13 of the 23 sera from the Addison patients studied contained antibodies which reacted with a 55 kDa adrenal microsomal protein. The same 13 sera were also positive for adrenal antibodies as judged by immunofluorescence. The 55 kDa protein was not immunoprecipitated from placenta or thyroid microsomes by Addison sera. Furthermore, patients with Graves' disease or rheumatoid arthritis did not immunoprecipitate the 55 kDa protein from adrenal microsomes. Our studies suggest therefore that Addison sera contain antibodies to a 55 kDa adrenal specific protein which may well be the antigen observed on immunofluorescence.