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Evidence that stimulation of gluconeogenesis by fatty acid is mediated through thermodynamic mechanisms
Author(s) -
Berry M.N.,
Gregory R.B.,
Grivell A.R.,
Henly D.C.,
Phillips J.W.,
Wallace P.G.,
Welch G.R.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80694-x
Subject(s) - gluconeogenesis , phosphoenolpyruvate carboxykinase , stimulation , chemistry , biochemistry , glycolysis , palmitic acid , fatty acid , endocrinology , medicine , biology , metabolism , enzyme
We have studied the stimulatory effects of palmitate on the rate of glucose synthesis from lactate in isolated hepatocytes. Control of the metabolic flow was achieved by modulating the activity of enolase using graded concentrations of fluoride. Unexpectedly, palmitate stimulated gluconeogenesis even when enolase was rate‐limiting. This stimulation was also observed when the activities of phospho enol pyruvate carboxykinase and aspartate aminotransferase were modulated using graded concentrations of quinolinate and aminooxyacetate, respectively. Linear force‐flow relationships were found between the rate of gluconeogenesis and indicators of cellular energy status (i.e. mitochondrial membrane and redox potentials and cellular phosphorylation potential). These findings suggest that the fatty acid stimulation of glucose synthesis is in part mediated through thermodynamic mechanisms.