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Observation of mobile poly(β‐hydroxybutyrate) in the storage granules of Methylobacterium AM1 by in vivo 13 C‐NMR spectroscopy
Author(s) -
Barnard Glenn N.,
Sanders Jeremy K.M.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80693-8
Subject(s) - trehalose , poly 3 hydroxybutyrate , nuclear magnetic resonance spectroscopy , granule (geology) , chemistry , in vivo , polymer , methylobacterium , spectroscopy , crystallography , materials science , stereochemistry , biochemistry , biology , organic chemistry , physics , microbiology and biotechnology , 16s ribosomal rna , quantum mechanics , gene , composite material
13 C‐NMR spectra of whole native cells of Methylobacterium AM1 show dominant signals belonging to poly(β‐hydroxybutyrate) and trehalose. Fractionation of the cells demonstrates that the poly(β‐hydroxybutyrate) is located in the storage granules and that the trehalose is located in the cytosol. The observation of relatively sharp poly(β‐hydroxybutyrate) signals indicates that the polymer granule in this organism is in a mobile rather than truly solid state in vivo.