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Quantitation and identification of carbon‐13 isotopomers by homonuclear polarization transfer NMR experiments
Author(s) -
Boudot Denis,
Canet Daniel,
Brondeau Jean,
Martin Francis
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80692-6
Subject(s) - isotopomers , homonuclear molecule , chemistry , polarization (electrochemistry) , coupling constant , pulse sequence , analytical chemistry (journal) , nuclear magnetic resonance , molecule , physics , chromatography , organic chemistry , particle physics
The simplest pulse sequence (π/2) x —τ— (π/2) y (Acquisition) for nuclear spin polarization transfer is used in a selective fashion: transfer only flows from a nucleus A to any nucleus X J ‐coupled to A, provided that the carrier frequency coincides with the A resonance and that τ is slightly varied around a properly chosen value, relevant free induction decays being co‐added. According to the value selected for τ (1/2 J or 1/4 J , J: one bond carbon‐carbon coupling constant), it proves possible to sort out and compare subspectra pertaining to isotopomers involving one, two, three, and four consecutive 13 C. The method has been applied to an extract of mycelium grown on a medium containing 13 C‐labeled acetate, with the aim of delineating metabolic pathways from proportions of the detected isotopomers.