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Human interferon‐γ lacking 23 COOH‐terminal amino acids is biologically active
Author(s) -
Sakaguchi Masao,
Honda Susumu,
Ozawa Mariko,
Nishimura Osamu
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80671-9
Subject(s) - dimer , circular dichroism , escherichia coli , amino acid , in vitro , chemistry , interferon , biological activity , biochemistry , stereochemistry , biology , gene , virology , organic chemistry
We constructed five mutated cDNAs encoding human interferon‐γ (IFN‐γ) derivatives lacking 19–23 COOH‐terminal residues and expressed them in Escherichia coli . All the derivatives were purified to homogeneity. They showed substantially the same order of antiviral activity in vitro as the intact molecule, and behaved as a dimer. The far‐ and near‐UV circular dichroism spectra of the derivatives were quite similar to those of the intact one. These results indicate that the 23 COOH‐terminal amino acids at least are not essential for achieving the full antiviral activity and constructing the higher structure of human IFN‐γ.