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Bleomycin resistance conferred by a drug‐binding protein
Author(s) -
Gatignol Anne,
Durand Henri,
Tiraby Gérard
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80665-3
Subject(s) - bleomycin , recombinant dna , in vitro , gene , microbiology and biotechnology , dna , biology , chemistry , biochemistry , genetics , chemotherapy
The protein coded by a bleomycin‐resistance gene ( ble ) cloned from producing actinomycetes was purified from a culture of a recombinant E.coli strain and its action on bleomycin was determined by in vitro assays. The protein binds reversibly in a one to one ratio to bleomycin which can no longer cleave DNA. The bleomycin resistance of cells harboring a ble gene could be accounted for by a sequestering effect of the bleomycin‐binding protein.