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The presumption of proteolytic enzymes related to the formation of intermediates during the terminal differentiation
Author(s) -
Katagata Yohtaro,
Aso Kazuo
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80660-4
Subject(s) - keratin , epidermis (zoology) , enzyme , chemistry , biochemistry , ammonium sulfate , ammonium , proteolytic enzymes , human skin , fraction (chemistry) , chromatography , biology , organic chemistry , anatomy , paleontology , genetics
The [ 14 C]Gly‐labelled keratin polypeptides extracted with 1% SDS and 10 mM DTT were made to undergo changes with an enzyme fraction (ammonium sulfate, 50–75% saturated fraction) prepared from a human epidermis in the presence of 1% Triton X‐100. In particular, 69‐67 kDa peptides were considerably decreased with the above enzyme fraction in the time course experiments, and the components strongly bound to the cell membrane had little effect on the above reaction. In addition, in the case of the [ 14 C]Gly‐labelled keratin filament assembly, 69 and 62 kDa peptides were decreased and 55, 52 and 50 kDa peptides were increased with the same enzyme fraction in the time course experiments. From these results, we estimated that the proteolytic enzyme(s) may exist in the human epidermis, and may be processed to keratin intermediates from prekeratin during the initial stage of terminal differentiation in the human epidermis.