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Biosynthesis of sialosyllactotetraosylceramide in human colorectal carcinoma cells
Author(s) -
Jolif Alain,
Liepkans Vis
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80659-8
Subject(s) - sialidase , sialic acid , biochemistry , carcinoma , enzyme , colorectal cancer , cell culture , chemistry , biosynthesis , biology , microbiology and biotechnology , cancer , neuraminidase , genetics
A monosialoganglioside, IV 3 ‐NeuNAcLcOse 4 Cer, has recently been detected in colorectal carcinoma cells, small cell lung carcinoma cells, embryonal carcinoma cells and in human brain extracts. We report here the presence of a CMP‐sialic: LcOse 4 Cer sialyl transferase activity in subcellular membrane fractions of the human colorectal carcinoma, SW1116, which recognizes the non‐reducing terminal galactosyl moiety of lactotetraosylceramide. A convenient method for structural analysis of picomolar quantities of the radioactive enzymatic product(s) using bacterial endoglycoceramidase, sialidase and a viral sialidase is presented.