Premium
Biosynthesis of very long chain monounsaturated fatty acids by subcellular fractions of developing seeds
Author(s) -
Murphy Denis J.,
Mukherjee Kumar D.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80650-1
Subject(s) - sinapis , biochemistry , erucic acid , chemistry , biosynthesis , fraction (chemistry) , composition (language) , fatty acid , food science , biology , botany , chromatography , brassica , enzyme , linguistics , philosophy
Subcellular fractions from developing seeds of mustard ( Sinapis alba ), honesty ( Lunaria annua ) and nasturtium ( Tropaeolum majus ) synthesize very long chain cis ( n −9) monounsaturated fatty acids, e.g. gadoleic (20:1), erucic (22:1) and nervonic (24:1) acid, from oleoyl‐CoA and malonyl‐CoA by condensation reactions. The particulate 2000 × g and 15 000 × g fractions exhibit considerably higher elongase activities compared to the microsomal or oil body fractions, whereas the soluble (150 000 × g supernatant) fraction is devoid of such activities.