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Antibodies against the human cellular 3,3′,5‐triiodo‐L‐thyronine‐binding protein (p58)
Author(s) -
Obata Toru,
Fukuda Takaaki,
Cheng Sheue-yann
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80630-6
Subject(s) - antibody , immunoprecipitation , antigen , titer , immune system , hormone , triiodothyronine , microbiology and biotechnology , biology , immunology , chemistry , medicine , endocrinology
High‐titer antibodies against a cellular thyroid hormone‐binding protein ( M r 58 000, p58) were developed by a special immunization method. To enhance immune responses, this method uses a boosting protocol in which repeated injections of small amounts of antigen are administered at 2‐day intervals. Antibodies were detected 1 week after the last injection of antigen by ELISA, Western dot blotting and immunoprecipitation. The anti‐p58 antibodies recognize p58 which is bound to the thyroid hormone. With the availability of anti‐p58 antibodies, it has become possible to study cellular localization and function.