Premium
Isolation of human pancreastatin fragment containing the active sequence from a glucagonoma
Author(s) -
Sekiya K.,
Ghatei M.A.,
Minamino N.,
Bretherton-Watt D.,
Matsuo H.,
Bloom S.R.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80606-9
Subject(s) - chromogranin a , glucagonoma , peptide sequence , peptide , sequence (biology) , protein primary structure , biology , biochemistry , chemistry , glucagon , immunohistochemistry , immunology , hormone , gene
The primary structure of a human pancreastatin‐like peptide was determined from a pancreatic glucagonoma. The 28‐amino acid peptide was identified using a specific antibody raised against porcine pancreastatin 1–49 and showed a 75% sequence homology with porcine pancreastatin 22–49 and bovine chromogranin A 267–294. Several forms of pancreastatin‐like immunoreactivity were found in human endocrine tumors of which the purified peptide was the smallest and contained the active sequence of pancreastatin.