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Monoclonal antibody to human endothelial cell surface internalization and liposome delivery in cell culture
Author(s) -
Trubetskaya O.V.,
Trubetskoy V.S.,
Domogatsky S.P.,
Rudin A.V.,
Popov N.V.,
Danilov S.M.,
Nikolayeva M.N.,
Klibanov A.L.,
Torchilin V.P.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80601-x
Subject(s) - biotinylation , internalization , monoclonal antibody , liposome , avidin , cell , endothelial stem cell , antibody , cell culture , biotin , monoclonal , microbiology and biotechnology , chemistry , medicine , immunology , biology , biochemistry , in vitro , genetics
A monoclonal antibody (mAb), E25, is described that binds to the surface of cultured human endothelial cells. Upon binding E25 is rapidly internalized and digested intracellularly. Selective liposome targeting to the surface of the cells is performed using a biotinylated E25 antibody and an avidin‐biotin system. Up to 30% of the cell‐adherent liposomal lipid is internalized.