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The primary structure of human platelet profilin: Reinvestigation of the calf spleen profilin sequence
Author(s) -
Ampe Christophe,
Markey Francis,
Lindberg Uno,
Vandekerckhove Joël
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80575-1
Subject(s) - profilin , sequence (biology) , spleen , protein primary structure , biology , chemistry , microbiology and biotechnology , genetics , peptide sequence , immunology , gene , actin cytoskeleton , cytoskeleton , cell
The primary structure of human platelet profilin was determined by aligning the sequences of its tryptic peptides to the previously determined calf spleen profilin sequence [(1979) FEBS Lett. 101, 161–165]. Comparison of the peptide fingerprints of the two proteins suggested a higher homology than that found by direct sequence comparison. We therefore reinvestigated the sequences of the peptides from calf spleen profilin. We identified four incorrect charge assignments and a deletion of three residues. The similarity between the two vertebrate profilins amounts to 95%.