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An initiator protein for plasmid R6K DNA replication Mutations affecting the copy‐number control
Author(s) -
Inuzuka Manabu,
Wada Yukie
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80573-8
Subject(s) - threonine , plasmid , mutation , mutant , genetics , dna replication , biology , microbiology and biotechnology , serine , dna , isoleucine , gene , amino acid , leucine , phosphorylation
Two kinds of mutations affecting the copy‐number control of plasmid R6K were isolated and identified in an initiator π protein by DNA sequencing. Firstly, a temperature‐sensitive replication mutation, ts22 , with decreased copy number results in a substitution of threonine to isoleucine at position 138 of the 305‐amino‐acid π protein. Secondly, a high‐copy‐number (cop21) mutant was isolated from this ts mutant and was identified by an alteration of alanine to serine at position 162. This cop21 mutation suppressed the Ts character and was recessive to the wild‐type allele in the copy control.