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Phosphohistidine is found in basic nuclear proteins of Physarum polycephalum
Author(s) -
Pesis Karen H.,
Wei Yingfei,
Lewis Matthew,
Matthews Harry R.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80563-5
Subject(s) - physarum polycephalum , physarum , biochemistry , histone , histidine , slime mold , biology , chemistry , amino acid , dna
Nuclear extracts of the true slime mold, Physarum polycephalum , show protein histidine kinase activity towards exogenous histones [(1985) J. Biol. Chem. 260, 16106–16113]. Physarum microplasmodia were labeled with [ 32 P]phosphate in vivo and two basic proteins containing alkali‐stable phosphate were detected. The labeled proteins comigrated with Physarum histones H1 (approximately) and H2A and phosphoamino acid analysis showed that each protein contained [ 32 P]phosphohistidine. The H2A‐like protein was also labeled in isolated nuclei incubated with [ 35 S]thio‐ATP. We conclude that some Physarum nuclear proteins contain phosphohistidine.