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Expression and electrophysiological identification of the receptor for bombesin and gastrin‐releasing peptide in Xenopus laevis oocytes injected with polyA + RNA from rat brain
Author(s) -
Meyerhof Wolfgang,
Morley Steven D.,
Richter Dietmar
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80555-6
Subject(s) - bombesin , xenopus , gastrin releasing peptide , medicine , peptide , endocrinology , receptor , electrophysiology , biology , neuropeptide , antagonist , voltage clamp , salientia , chemistry , microbiology and biotechnology , biochemistry , gene
The receptor for bombesin and the related peptide, gastrin‐releasing peptide (GRP) has been induced in frog oocytes by injection of polyA + RNA from rat brain. The primed oocytes responded to peptides of the bombesin family (GRP, neuromedin C of bombesin) by showing dose‐dependent oscillations in membrane currents as recorded by the voltage‐clamp method. The induced membrane changes were suppressed when oocytes were pretreated with a bombesin‐receptor antagonist.