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Spectroscopic characterization of reaction center crystals from the carotenoid‐containing wild‐type strain Rhodobacter sphaeroides Y
Author(s) -
Reiss-Husson F.,
Mäntele W.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80549-0
Subject(s) - rhodobacter sphaeroides , bacteriochlorophyll , photosynthetic reaction centre , chemistry , crystallography , linear dichroism , crystal (programming language) , circular dichroism , photochemistry , electron donor , electron acceptor , dichroism , electron transfer , photosynthesis , pigment , optics , organic chemistry , physics , biochemistry , computer science , programming language , catalysis
Thin three‐dimensional crystals of the photochemical reaction center from Rhodobacter sphaeroides wild‐type strain Y were investigated with microspectrophotometric methods. Absorbance and linear dichroism spectra were obtained for two crystal forms with the same type of unit cell, but grown in different morphological forms. High dichroism was found for the Q x and the Q y transitions of the bacteriopheophytin as well as for the Q y transitions of the accessory bacteriochlorophylls and the bacteriochlorophylls forming the primary electron donor. Low absorption and dichroism of the carotenoid transitions in the plane of the crystal suggests a large component out of the crystal plane. Photoactivity of the crystal was demonstrated by light‐induced charge separation. The analysis of the rates of charge recombination indicates that almost all reaction centers in the crystal (> 90%) contain the secondary electron acceptor Q B .