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Tumor necrosis factor stimulates prostaglandin production and cyclic AMP levels in rat cultured mesangial cells
Author(s) -
Baud Laurent,
Perez Joelle,
Friedlander Gérard,
Ardaillou Raymond
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80543-x
Subject(s) - tumor necrosis factor alpha , endocrinology , chemistry , medicine , tumor necrosis factor α , mesangial cell , cyclic gmp , prostaglandin e2 , prostaglandin , prostaglandin e , necrosis , microbiology and biotechnology , biology , kidney , nitric oxide
Human recombinant tumor necrosis factor‐α (TNF) was found to stimulate the production of prostaglandins (PG) by cultured rat mesangial cells. This effect was demonstrable from 6 h, was dose dependent and affected the synthesis of PGE 2 , PGF 2α , and 6‐keto‐PGF 1α . It required both RNA and protein synthesis but was not associated with a modification of cell proliferation. TNF also stimulated adenosine 3′–5′ cyclic monophosphate (cAMP) levels in the mesangial cell culture medium. Indomethacin suppressed the effect of TNF on PGs but only reduced that on cAMP, indicating that PG production partly mediates the increase in cAMP. These findings demonstrate that mesangial cells can be a target for TNF and that the mechanism of TNF action includes stimulation of both PG production and cAMP levels.