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Cloning a synthetic gene for human stefin B and its expression in E. coli
Author(s) -
Jerala Roman,
Trstenjak Mojca,
Lenarčič Brigita,
Turk Vito
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80541-6
Subject(s) - cloning (programming) , recombinant dna , fusion protein , microbiology and biotechnology , papain , molecular cloning , escherichia coli , gene , insert (composites) , chemistry , expression vector , coding region , biology , peptide sequence , biochemistry , enzyme , computer science , engineering , programming language , mechanical engineering
A gene coding for human stefin B was synthesized by the solid‐phase phosphite method and cloned in the pUC8 cloning vector. The insert with the verified DNA sequence was subcloned into two expression vectors and expressed in E. coli as a fusion protein with β‐galactosidase and as a native protein. The CNBr cleaved fusion protein and the native recombinant stefin B were inhibitory to papain and reacted with antibodies against human stefin B.