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Electrogenic photocycle of the 13‐ cis retinal‐containing bacteriorhodopsin with an M intermediate involved
Author(s) -
Drachev L.A.,
Kaulen A.D.,
Skulachev V.P.,
Zorina V.V.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80533-7
Subject(s) - bacteriorhodopsin , chemistry , retinal , halobacteriaceae , chromophore , proton , solubilization , retinaldehyde , photochemistry , crystallography , membrane , biophysics , stereochemistry , biochemistry , halobacterium salinarum , rhodopsin , biology , physics , quantum mechanics
It has been found that bacteriorhodopsin containing 13‐ cis ‐retinal as the chromophore (13‐ cis ‐bR) is competent in a one‐photon photocycle which involves an M‐type intermediate. Such an effect can be shown in 13‐ cis ‐Br obtained by means of 13‐ cis ‐retinal treatment of white bacterioopsin membrane sheets, as well as in dark‐adapted purple sheets, solubilized Br and Br proteoliposomes. The 13‐ cis ‐Br photocycle with the M intermediate is found to be proton‐motive, the electrogenic phases resembling those of the all‐ trans ‐Br photocycle. All of these effects are observed only when the Ph is above some critical value, namely Ph 8.5–9.5 for the Br sheets, 7.6 for proteoliposomes and 7.0 for the solubilized Br.