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Expression of four types of human tyrosine hydroxylase in COS cells
Author(s) -
Kobayashi Kazuto,
Kiuchi Kazutoshi,
Ishii Akira,
Kaneda Norio,
Kurosawa Yoshikazu,
Fujita Keisuke,
Nagatsu Toshiharu
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80526-x
Subject(s) - tyrosine hydroxylase , tyrosine , enzyme , biochemistry , chemistry , alternative splicing , tyrosine 3 monooxygenase , gene expression , gene , rna splicing , biology , microbiology and biotechnology , messenger rna , rna
Alternative splicing from a single gene produces four kinds of human tyrosine hydroxylase (types 1–4) which have structural diversity only in the N‐terminal region. We attempted expression of the type 1–4 enzymes in COS cells and performed kinetic analyses. All had enzymatic activities. The K m values of the four types for L‐tyrosine and 6‐methyl‐5,6,7,8‐tetrahydropteridine were similar, although their relative homospecific activities were clearly different. The type 1 enzyme displayed the highest activity.