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Interaction of a hydrophobic model peptide and its fatty acid derivative with lipid vesicles
Author(s) -
Joseph M.,
Nagaraj R.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80522-2
Subject(s) - chemistry , vesicle , peptide , derivative (finance) , lipid vesicle , fatty acid , biochemistry , membrane , financial economics , economics
The interaction of a peptide [Lys‐Phe‐Phe‐Phe‐Ile‐Ile‐Trp‐OCH 3 ] and its fatty acid derivatives [Lys‐(ε‐palmitoyl)‐Phe‐Phe‐Phe‐Ile‐Ile‐Trp‐OCH 3 and Lys‐{ε‐12‐(9‐anthroyloxy)stearic acid}‐Phe‐Phe‐Phe‐Ile‐Ile‐OCH 3 ] with model membranes was investigated by fluorescence spectroscopy. The emission characteristics of the Trp fluorophore indicated that only the peptide with the fatty acid chain is associated with lipid vesicles. Quenching experiments with spin probes suggest an orientation for the fatty acylated peptide wherein the fatty acid chain is perpendicular to the bilayer surface and the peptide chain parallel to the bilayer surface.