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400‐MHz 1 H‐NMR spectroscopy of fucosylated tetrasialyl oligosaccharides isolated from normal and cirrhotic α 1 ‐acid glycoprotein
Author(s) -
Wieruszeski J.M.,
Fournet B.,
Konan D.,
Biou D.,
Durand G.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80518-0
Subject(s) - fucose , oligosaccharide , glycoprotein , chemistry , nuclear magnetic resonance spectroscopy , spectroscopy , biochemistry , stereochemistry , physics , quantum mechanics
The comparative study of fucosylated tetrasialyl‐oligosaccharides isolated from normal and cirrhotic α 1 ‐AGP was performed using permethylation and 400‐MHz 1 H‐NMR spectroscopy. These results clearly show the tetraantennary structure of these two oligosaccharides with hyperfucosylation for the tetrasialylated fraction from cirrhotic α 1 ‐AGP. In the latter oligosaccharide the simultaneous presence on two antennae (7 and 7′) of the sialosyl Lewis X determinant NeuAc‐(α2–3) Gal(β1–4) [Fuc(αl–3)l GlcNAc has been observed. Moreover the 5 and 5′ antennae were α2–6 sialylated but without fucose.