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Low‐density lipoproteins increase intracellular calcium in aequorin‐loaded platelets
Author(s) -
Dunn R.C.,
Schachter M.,
Miles C.M.M.,
Feher M.D.,
Tranter P.R.,
Bruckdorfer K.R.,
Sever P.S.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80512-x
Subject(s) - aequorin , chemistry , extracellular , intracellular , calcium , platelet , calcium in biology , photoprotein , biochemistry , biophysics , egta , guanosine , low density lipoprotein , medicine , cholesterol , biology , organic chemistry
Low‐density lipoproteins activate isolated human platelets. The mechanism of this activation is unknown, but may involve increased phosphoinositide turnover. We have examined the effect of low‐density lipoproteins on intracellular calcium concentrations in platelets loaded with the photoprotein aequorin. The lipoproteins induced concentration‐dependent increases in intracellular calcium, associated with shape change and aggregation. These responses could be partially inhibited by the removal of extracellular calcium and by pre‐incubation with acetylsalicylic acid. They were also antagonised by agents which increase cellular concentrations of cyclic adenosine and guanosine monophosphates. It is not clear whether the platelet‐lipoprotein interaction involves a ‘classical’ lipoprotein receptor.