Premium
Inhibition of adriamycin‐promoted microsomal lipid peroxidation by β‐carotene, α‐tocopherol and retinol at high and low oxygen partial pressures
Author(s) -
Vile Glenn F.,
Winterbourn Christine C.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80511-8
Subject(s) - lipid peroxidation , carotene , tocopherol , chemistry , retinol , microsome , beta carotene , carotenoid , oxygen , biochemistry , vitamin e , antioxidant , food science , vitamin , organic chemistry , enzyme
Iron‐dependent peroxidation of rat liver microsomes, enhanced by adriamycin, was measured in the presence of increasing concentrations of α‐tocopherol, β‐carotene and retinol at low and high p O 2 . β‐Carotene and α‐tocopherol inhibited lipid peroxidation by more than 60% when present at concentrations greater than 50 nmol/mg microsomal protein at both high and low p O 2 . Retinol inhibited peroxidation by 39% at concentrations greater than 100 nmol/mg microsomal protein. This maximal level of inhibition by retinol was unaltered by p O 2 . However, β‐carotene was more effective than α‐tocopherol or retinol at a p O 2 of 4 mmHg, whereas α‐tocopherol was more effective under aerobic conditions. Since adriamycin‐dependent lipid peroxidation is maximal at low p O 2 , β‐carotene may play a role in protecting against this process.