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Cloning and high‐level expression of a chloroperoxidase gene from Pseudomonas pyrrocinia in Escherichia coli
Author(s) -
Wolfframm Christina,
van Pée Karl-Heinz,
Lingens Franz
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80505-2
Subject(s) - cosmid , escherichia coli , microbiology and biotechnology , plasmid , pseudomonas , gene , cloning (programming) , biology , molecular cloning , expression vector , chemistry , gene expression , genetics , recombinant dna , bacteria , computer science , programming language
A chloroperoxidase gene from Pseudomonas pyrrocinia was cloned into Escherichia coli using the cosmid vector pJB8. The gene coding for the chloroperoxidase could be localized to a 1.5 kb fragment of DNA which was subcloned into the high‐copy‐number plasmid pUC18. In one subclone increased halogenating activity could be found which was 570‐fold greater than in P. pyrrocinia . The halogenating enzyme was identified as the chloroperoxidase by SDS‐polyacrylamide gel electrophoresis.