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Botulinum toxin‐induced ADP‐ribosylation and inhibition of exocytosis are unrelated events
Author(s) -
Adam-Vizi V.,
Rosener S.,
Aktories K.,
Knight D.E.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80496-4
Subject(s) - adp ribosylation , intracellular , exocytosis , neurotoxin , toxin , secretion , inhibitory postsynaptic potential , chemistry , biology , biochemistry , enzyme , endocrinology , nad+ kinase
The hypothesis that inhibition of secretion by botulinum neurotoxin type D occurs by an intracellular process involving ADP‐ribosylation has been directly tested by measuring both the extent of inhibition of secretion and of ADP‐ribosylation in the same cells. Although the inhibitory effect of unpurified toxin closely parallels intracellular ribosylation, the two events are clearly unrelated, as using purified D and C3 toxins together with their antibodies, each of these events can be either stimulated or inhibited independently of each other.