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Isolation and analysis of the mouse opsin gene
Author(s) -
Baehr W.,
Falk J.D.,
Bugra K.,
Triantafyllos J.T.,
McGinnis J.F.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80490-3
Subject(s) - opsin , biology , complementary dna , coding region , microbiology and biotechnology , intron , gene , rna , cdna library , retina , genetics , gene expression , rhodopsin , retinal , biochemistry , neuroscience
We have identified three overlapping 5′‐truncated mouse opsin cDNA clones by immunologically screening a λgt11 retina expression library. Using one of the cDNA clones as a probe, we isolated a 5 kb genomic fragment that encompassed the complete coding sequence for mouse opsin. The coding region for opsin was interrupted by four introns positioned precisely as those previously described for other mammalian opsins. In contrast to the single major opsin mRNA in the bovine and human retina, Northern analysis of mouse retina RNA demonstrated the presence of at least five distinct species of polyadenylated opsin mRNAs. Their sizes ranged from 1.7 kb to 5.1 kb.