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Vitamin E and selenium regulate balance between β‐adrenergic and muscarinic responses in rat lungs
Author(s) -
Doelman C.J.A.,
Kramer K.,
Timmerman H.,
Bast A.
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80476-9
Subject(s) - muscarinic acetylcholine receptor , adrenergic , endocrinology , chemistry , vitamin e , medicine , selenium , biochemistry , antioxidant , receptor , organic chemistry
The effects of hydrogen peroxide on the β‐adrenergic and muscarinic responses of the rat trachea muscle were studied in vitro, after feeding rats, for 6 weeks, either a diet deficient in vitamin E and selenium or a control diet. In the control situation after incubation with 1 mM hydrogen peroxide for 30 min, a reduction of the maximal response to methacholine of 39% occurred whereas no p D 2 shift could be demonstrated. Moreover, no response to isoprenaline after precontraction with 3 × 10 −7 M methacholine was left. In the deficient situation, we found a reduction to 64% of the response to methacholine after incubation with 1 mM hydrogen peroxide. Again isoprenaline became inactive, i.e. no relaxation with isoprenaline was observed after precontraction with 3 × 10 −7 M methacholine. We therefore conclude that vitamin E and selenium protect against oxidative stress in lung tissue and thus regulate the (patho‐) physiological balance between adrenergic and muscarinic responses.