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Is the acetylcholine releasing protein mediatophore present in rat brain?
Author(s) -
Israe¨l Maurice,
Lesbats Bernard,
Morel Nicolas,
Manaranche Robert,
Le Gal la Salle Gildas
Publication year - 1988
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(88)80475-7
Subject(s) - torpedo , membrane , acetylcholine , cholinergic , chemistry , cerebellum , free nerve ending , acetylcholinesterase , biochemistry , calcium , biophysics , electric organ , microbiology and biotechnology , biology , neuroscience , acetylcholine receptor , anatomy , pharmacology , enzyme , receptor , organic chemistry
Mediatophore is a protein purified from the nerve terminal membranes of Torpedo electric organ. It confers to artificial membranes a calcium‐dependent mechanism that translocates acetylcholine. When similar reconstitution experiments are applied to rat brain synaptosomal membranes they reveal the presence of mediatophore activity with properties close to those described for the Torpedo protein (extractability, sensitivity to calcium, and effect of the drug cetiedil). The acitivity was more abundant in synaptosomal membranes than in mitochondrial or myelinic membranes and in cholinergic areas as compared to cerebellum.